Volume 8, Issue 4 (12-2018)
J Health Saf Work 2018, 8(4): 359-370 |
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Ramin Sabet M, Pourhossein M, Khadem M, Omidi F, Golbabaei F, Shahtaheri S J. Development of dispersive liquid-liquid micro-extraction procedure for trace determination of pesticide diazinon in urine samples. J Health Saf Work 2018; 8 (4) :359-370
URL: http://jhsw.tums.ac.ir/article-1-5959-en.html
URL: http://jhsw.tums.ac.ir/article-1-5959-en.html
Maryam Ramin Sabet1 
, Mehran Pourhossein1 , Monireh Khadem2 , Fariborz Omidi3 , Farideh Golbabaei4 , Seyed Jamaleddin Shahtaheri * 5
, Mehran Pourhossein1 , Monireh Khadem2 , Fariborz Omidi3 , Farideh Golbabaei4 , Seyed Jamaleddin Shahtaheri * 5
1- Ph.D., Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2- Assistant Professor, Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3- Ph.D., Research Center for Environmental Determinants of Health (RCEDH), Kermanshah University of Medical Sciences, Kermanshah, Iran
4- Professor, Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5- Professor, Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , shahtaheri@tums.ac.ir
2- Assistant Professor, Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3- Ph.D., Research Center for Environmental Determinants of Health (RCEDH), Kermanshah University of Medical Sciences, Kermanshah, Iran
4- Professor, Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5- Professor, Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , shahtaheri@tums.ac.ir
Abstract: (4299 Views)
Introduction: Measurement of pesticides in biological matrices is become a serious challenge for researchers due to their very low concentration in different matrices. The aim of this study was to develop a new sample preparation method with high accuracy and validity and short retention time for determination of diazinon.
Material and Method: Dispersive liquid-liquid micro-extraction technique coupled with high performance liquid chromatography equipped with ultra violet detector was developed for trace extraction and determination of diazinon pesticide in human urine samples. One variable at a time method was used to optimize parameters affecting the diazinon extraction. Different parameters such as extraction solvent, disperser solvent, and volume of the extraction solvent, volume of the disperser solvent, centrifugation time and speed, salt addition, and sample pH were studied and optimized.
Result: Findings showed that optimal levels of these variables for diazinon pesticide were 150 μl of carbon tetrachloride as extraction solvent, 1.5 ml of methanol as dispersive solvent, pH of 6, 5 minutes centrifuge time at speed of 4000 rpm , 0% (w/v) salt addition. Correlation coefficient was 0.9965 indicating the linearity of a wide range of concentrations of the toxin. LOD and LOQ was calculated less than 0.7 and 5 µg L-1 respectively. The relative standard deviation for six replicate experiments in intra-day and inter-day at tree selected concentrations (50, 200 and 1000 µg L-1) was less than 4% that indicates the accuracy and precision of the optimized method. Enrichment factor and extraction recovery for diazinon were 245 and 99% respectively.
Conclusion: According to the results, dispersive liquid-liquid micro-extraction procedure was successfully developed for the extraction of diazinon from human urine samples. Compared to other extraction techniques, the proposed procedure had some advantages such as shorter extraction time, better reproducibility, and higher enrichment factor.
Material and Method: Dispersive liquid-liquid micro-extraction technique coupled with high performance liquid chromatography equipped with ultra violet detector was developed for trace extraction and determination of diazinon pesticide in human urine samples. One variable at a time method was used to optimize parameters affecting the diazinon extraction. Different parameters such as extraction solvent, disperser solvent, and volume of the extraction solvent, volume of the disperser solvent, centrifugation time and speed, salt addition, and sample pH were studied and optimized.
Result: Findings showed that optimal levels of these variables for diazinon pesticide were 150 μl of carbon tetrachloride as extraction solvent, 1.5 ml of methanol as dispersive solvent, pH of 6, 5 minutes centrifuge time at speed of 4000 rpm , 0% (w/v) salt addition. Correlation coefficient was 0.9965 indicating the linearity of a wide range of concentrations of the toxin. LOD and LOQ was calculated less than 0.7 and 5 µg L-1 respectively. The relative standard deviation for six replicate experiments in intra-day and inter-day at tree selected concentrations (50, 200 and 1000 µg L-1) was less than 4% that indicates the accuracy and precision of the optimized method. Enrichment factor and extraction recovery for diazinon were 245 and 99% respectively.
Conclusion: According to the results, dispersive liquid-liquid micro-extraction procedure was successfully developed for the extraction of diazinon from human urine samples. Compared to other extraction techniques, the proposed procedure had some advantages such as shorter extraction time, better reproducibility, and higher enrichment factor.
Type of Study: Research |
Received: 2018/12/7 | Accepted: 2018/12/7 | Published: 2018/12/7
Received: 2018/12/7 | Accepted: 2018/12/7 | Published: 2018/12/7
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